Electrochemical biosensing strategies for DNA methylation analysis

dna-directed methylation analysis methodology

epigenetics alterations, especially dna methylation, play a critical role in control of gene expressions. abnormal patterns of methylation are observed in early-stages of many cancers. therefore, methylation analysis is useful in primary detection of tumors. advances knowledge about the functional role of aberrant epigenetic modifications as potential biomarkers for cancer, have attracted consi...

Electrochemical biosensors for DNA analysis.

Over the past few years, progress in electrochemical biosensors for DNA analysis is outstanding. This article briefly reviewed the principals of such biosensors, followed by a selection of topics that are of particular current interest. Selectivity and sensitivity are the two major challenges in DNA analysis. The former can be accomplished using peptide nucleic acids as probes, or relying on th...

Electrochemical Biosensing with Carbon Nanotubes

Graphene-supported ferric porphyrin as a peroxidase mimic for electrochemical DNA biosensing.

A novel peroxidase mimic was designed by loading ferric porphyrin and streptavidin onto graphene, which was used to recognize a biotinylated molecular beacon for specific electrochemical detection of DNA down to attomolar levels.

DNA Methylation Analysis

Epigenetic mechanisms play important roles during normal development1, aging2, and a variety of disease conditions. Numerous studies have implicated aberrant methylation in the etiology of common human disease, including cancer, MS, diabetes, and schizophrenia3,4. Hypermethylation of CpG islands located in the promoter regions of tumor suppressor genes is firmly established as the most frequent...